Fig. 4.
Detection of platelet-bound fibrinogen in whole blood.
A volume (5 μL) of freshly drawn citrated blood was incubated for 20 minutes at RT with 6.3 μg rabbit FITC-conjugated polyclonal rabbit anti–human fibrinogen. Saturating concentrations of anti-FcγRII blocking antibody, IV.3, were added to prevent platelet activation through the Fc receptors by immune complexes formed between soluble fibrinogen and antifibrinogen antibody. In parallel, 5 μL whole blood was incubated with 5 μg/mL FITC-conjugated anti-GPIIb antibody SZ22 to assess the GPIIb-IIIa expression at the cell surface. The samples were analyzed by flow cytometry and expressed as the MFI. These data are representative of 4 separate experiments.
