Fig. 8.
Fig. 8. Flow cytometric analysis of LIBS exposure on surface-expressed GPIIb-Arg560IIIa. / (A) Transfected CHO cells were incubated with 20 μg/mL LIBS antibodies for 45 minutes at RT, followed by FITC-conjugated goat anti–mouse IgG. The binding of LIBS mAbs to GPIIb-IIIa was expressed as a LIBS index (LI) by normalizing the MFI of each LIBS antibody to that obtained using AP2, a complex-specific mAb the binding of which is unaffected by the Cys560Arg mutation (LI = LIBS mAb MFI/AP2 MFI). (B) Flow cytometric analysis of the binding of the activation-dependent fibrinogen-mimetic antibody PAC-1 (20 μg/mL) to GPIIb-Arg560IIIa (bold histogram) was performed in the presence of buffer (left), 2 mM RGDW (middle), or RGEW (right). Peptides were synthesized at the Peptide Core Laboratory of the Blood Research Institute (Milwaukee, WI). The MFIs are indicated on the histograms.

Flow cytometric analysis of LIBS exposure on surface-expressed GPIIb-Arg560IIIa.

(A) Transfected CHO cells were incubated with 20 μg/mL LIBS antibodies for 45 minutes at RT, followed by FITC-conjugated goat anti–mouse IgG. The binding of LIBS mAbs to GPIIb-IIIa was expressed as a LIBS index (LI) by normalizing the MFI of each LIBS antibody to that obtained using AP2, a complex-specific mAb the binding of which is unaffected by the Cys560Arg mutation (LI = LIBS mAb MFI/AP2 MFI). (B) Flow cytometric analysis of the binding of the activation-dependent fibrinogen-mimetic antibody PAC-1 (20 μg/mL) to GPIIb-Arg560IIIa (bold histogram) was performed in the presence of buffer (left), 2 mM RGDW (middle), or RGEW (right). Peptides were synthesized at the Peptide Core Laboratory of the Blood Research Institute (Milwaukee, WI). The MFIs are indicated on the histograms.

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