Expression of the internal pool of GPIIb-IIIa on the patient's platelets and subsequent aggregation after TRAP6 activation.
(A) A total of 2 × 106 platelets in PRP were activated with 50 μM TRAP6. Platelets were then incubated with anti–GPIIb-IIIa antibody 6E1 (clear histogram) and isotype control (dark histogram) for 30 minutes at RT. After the addition of 1% formaldehyde in PBS, samples were analyzed by flow cytometry. The MFIs are indicated on the histograms. (B) Aggregation of N.M.'s platelets in PRP after secretion of the internal GPIIb-IIIa pool. Citrated PRP (2 × 108platelets/mL) was stimulated with 7 μM and 50 μM TRAP6 for the control and the patient, respectively. The values (percent of maximal secretion) for PF4 release, as assessed with an ELISA, are in parentheses.
