Fig. 5.
Evaluation of the cytotoxic/cytostatic activity of SNP (1 mM), used alone or in combination with TRAIL (1 μg/mL) on K562.
After 24 hours of culture, viable cells were counted by trypan blue dye exclusion (A; data are expressed as percentage of His peptide–treated controls) and the percentage of apoptosis was quantitatively evaluated by flow cytometry after PI staining (B). In panels A and B, data represent the means ± SD of 5 independent experiments performed in duplicate. In panel C, the cell cycle was evaluated by PI DNA staining and flow cytometry. The insets show the percentage of cells with a G1(2n), S, G2/M(4n) DNA content, calculated excluding apoptotic cells (Apo). A representative analysis of 4 separate experiments is shown.
