Evaluation of the cytotoxicity of TRAIL, SNP, and SNP plus TRAIL on HEL, HL-60, Jurkat, SupT1, RPMI 8226, normal erythroblasts, and normal granulocytic cells.

After 24 hours of culture, viable cells were counted by trypan blue dye exclusion (A; data are expressed as percentage of His peptide controls) and the percentage of apoptosis was quantitatively evaluated by flow cytometry after PI staining (B). Data represent the means ± SDs of 4 independent experiments performed in duplicate.