Grb2, SHP-2, and Shc are associated with the PI3K complex in HS2 cells.

(A) Protein extracts from 663 HS1 and 606 HS2 cells were immunoprecipitated (IP) with anti-Grb2, anti-p85, and anti-Gab1 antibodies and analyzed by immunoblotting (WB) with anti-Grb2 antibody. The position of Grb2 is indicated. (B) Proteins immunoprecipitated from 663 HS1 and 606 HS2 cell lysates with anti-Grb2 or anti-p85 antibodies (IP) were analyzed by immunoblotting (WB) with anti-PY antibodies. (C) Proteins immunoprecipitated from 663 HS1 and 606 HS2 cell lysates with anti-Grb2 or anti-p85 antibodies (IP) were analyzed by immunoblotting (WB) with anti–SHP-2 and Shc antibodies. The positions of the phosphorylated proteins were indicated.