Abundant kinase activity of akt is conferred to H7 cells by gag-akt, but not RafCAAX expression.

Lysates of NIH 3T3 cells, treated or not with EGF, and lysates of H7RafCAAX cells or H7gag-akt/RafCAAX cells, both taken from their normal growth medium, were immunoprecipitated with akt antibody or control antibody (mock), and kinase assays were performed.