Fig. 3.
Effect of mAbs on adhesion of αMβ2-transfected cells to αEC and γC.
Calcein-labeled cells were preincubated with different concentrations of mAb 44a (A) or mAb IB4 (B) for 20 minutes at 22°C with constant agitation. Then, aliquots (0.1 mL) containing 5 × 104cells were added to the wells of microtiter plates coated with 5 μg/mL recombinant αEC (●) or γC (▾) domains. After 25 minutes at 37°C, nonadherent cells were removed and cell fluorescence was measured.
