Fig. 4.
Inhibition of cell adhesion to the immobilized αEC and D100 by P1 and P2-C peptides.
Calcein-labeled αMβ2-transfected cells were preincubated with increasing concentrations of P1 (open symbols) and P2-C (solid symbols) peptides for 20 minutes at 22°C. Aliquots (0.1 mL; 2.5 × 105 cells/mL) were added to wells coated with 10 μg/mL αEC (circles) or 2 μg/mL D100(triangles), and adhesion was quantitated. Values are the numbers of adherent cells. Data from a representative experiment are shown.