Adhesion of α_Xβ₂-expressing CHO cells to α_EC and D₁₀₀.

(A) Calcein-labeled cells (0.1 mL; 5 × 10⁵cells/mL) were added to the wells of 96-well microtiter plates coated with various concentrations of α_EC (●) and D₁₀₀ (▿) and postcoated with 1% PVP. After the cells were allowed to adhere for 25 minutes at 37°C, nonadherent cells were removed by 3 washes with phosphate-buffered saline and the number of adherent cells was determined by measuring the fluorescence. (B) Effect of mAbs and NIF on adhesion of cells to wells coated with 10 μg/mL α_EC. The cells were preincubated with 10 μg/mL mAb IB4, 10 μg/mL mAb 3.9, or 2 μg/mL NIF for 15 minutes at 22°C. Aliquots of cells were then added to wells coated with α_EC. Results are the total numbers of adherent cells and the mean ± SE values from 2 individual experiments.