Inhibition of gp120 binding to MDDCs with a range of ligands.

The mAbs to CD4 2-Leu3a and 3-Q4120), MR (4-clones 19 and 5-3.29), and DC-SIGN (AZN-5-D1 and 6-AZN-D2) were preincubated 5-fold above predetermined saturating concentrations (5 μg/mL). Inhibitors mannan (8-mannan), α-methyl-mannopyranoside (9-M/P), and EGTA (10-EGTA) were incubated in excess at 5 mg/mL, 125 mM, and 5mM, respectively. Dual Leu3a and mannan inhibition (11-2 and 8) included Leu3a and mannan at levels used above for treatments 2 and 8. Positive and negative controls (treatments 1 and 12) consisted of MDDCs incubated with or without gp120, respectively. The b-gp120 was added and detected as in the legend to Figure 1A.