Binding of gp120 on several DC subsets.

(A) Relative gp120 binding levels within blood DC subsets. Blood DCs were freshly isolated as outlined in “Materials and methods.” The b-gp120 (Leu3a) was added and then incubated and detected as outlined in Figure 1A. Cultured blood DCs were incubated as outlined in Figure 6. Blood DC subsets were further analyzed by CD11c staining after b-gp120 staining. (B) Inhibition of gp120 binding by anti-CD4 (Leu3a) and mannan to MDDCs and blood DCs. Inhibitors were preincubated with blood DCs as follows: (1) No inhibitors, (2) CD4 (Leu3a) (10 μg/mL), (3) mannan (5 mg/mL), and (4) dual mannan/Leu3a were preincubated with blood DCs. The b-gp120 was added as outlined in Figure 1A and DC subsets analyzed for gp120 binding after CD11c staining.