Affinity purification of recombinant human P-selectin (rhP-selectin) with immobilized PfEMP1.

Soluble rhP-selectin was incubated with bead-immobilized PfEMP1. Binding of rhP-selectin was detected by western blotting (lane 1), and the solubilized protein comigrated with rhP-selectin (lane 4). No band was seen with the extracts from the noninteracting parasite line D10 (lane 2) or noninfected erythrocytes (lane 3).