Fig. 2.
Gel-retardation assay of PfIRPa-mammalian IRE complexes.
Hemoglobin-free lysates from P falciparum–parasitized erythrocytes were incubated with radiolabeled mammalian consensus32P-IRE and were resolved on 8% nondenaturing polyacrylamide gel. Lane 1, consensus IRE alone (1.0 pmol/lane); 2 and 3, 5 μg P falciparum lysate protein plus IRE; 4 and 5, 5 μg lysate protein from hemoglobin-depleted uninfected erythrocytes, 6 and 7, 5 μg lysate protein from a human EBV cell line expressing IRP1. Visible traces of IRP1 on lanes 4 and 5 are probably from the reticulocyte precursors of the erythrocytes as judged on similar mobility with the bands on lanes 6 and 7. The boxed area shows the region of the gel that will be shown on the following figures.
