Fig. 1.
Fig. 1. Multiplex PCR products analyzed on 2% agarose gel. / The presence or absence of GSTM1 and GSTT1 genes was detected by the presence or absence of a band at 480 base pair (bp) (corresponding to GSTT1) and a band at 215 bp (corresponding to GSTM1). A band at 312 bp (corresponding to 1A1gene) was always present and was used as an internal control to document successful PCR amplification. Lanes 1,5, individuals withGSTT1+/+ andGSTM1+/+; lanes 2,7, individuals with GSTT1−/− andGSTM1+/+ alleles; lane 4, individuals with GSTT1+/+ andGSTM1−/− alleles; lanes 3,6,8, individuals with deletions for both GSTM1 andGSTT1.

Multiplex PCR products analyzed on 2% agarose gel.

The presence or absence of GSTM1 and GSTT1 genes was detected by the presence or absence of a band at 480 base pair (bp) (corresponding to GSTT1) and a band at 215 bp (corresponding to GSTM1). A band at 312 bp (corresponding to 1A1gene) was always present and was used as an internal control to document successful PCR amplification. Lanes 1,5, individuals withGSTT1+/+ andGSTM1+/+; lanes 2,7, individuals with GSTT1−/− andGSTM1+/+ alleles; lane 4, individuals with GSTT1+/+ andGSTM1−/− alleles; lanes 3,6,8, individuals with deletions for both GSTM1 andGSTT1.

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