Fig. 7.
Fig. 7. Interference of vIL-6 binding to sgp130 by neutralizing mAbs against vIL-6. / Biotinylated MBPvIL-6 (50 ng/mL) was first incubated with anti–vIL-6 mAbs (20 μg/mL) or isotype control mouse IgG1 (20 μg/mL), and then added onto ELISA wells coated with purified sgp130 (2 μg/mL) in triplicate. Bound protein was detected by streptavidin-HRP. The results represent the means ± SDs. The asterisk indicates the occurrence of a significant decrease in OD450/630(P < .002) compared to control antibody. Representative data of 2 independent experiments are shown.

Interference of vIL-6 binding to sgp130 by neutralizing mAbs against vIL-6.

Biotinylated MBPvIL-6 (50 ng/mL) was first incubated with anti–vIL-6 mAbs (20 μg/mL) or isotype control mouse IgG1 (20 μg/mL), and then added onto ELISA wells coated with purified sgp130 (2 μg/mL) in triplicate. Bound protein was detected by streptavidin-HRP. The results represent the means ± SDs. The asterisk indicates the occurrence of a significant decrease in OD450/630(P < .002) compared to control antibody. Representative data of 2 independent experiments are shown.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal