Donor chimerism induced after nonmyeloablative conditioning is associated with donor-specific CTL tolerance.

Spleen cells from B10 (▪) and B10.BR (▴) mice and from B10.BR→B10 chimeras generated after conditioning with Flu-XRT-Cy (●; as described in the legend to Figure 1) were cultured for 5 days with irradiated B10, B10.BR, or BALB/c stimulators and tested for killing of³H-labeled B10 (A), B10.BR (B), or BALB/c targets (C), respectively. Chimeras were tested 6 weeks after transplantation.