Fig. 3.
Fig. 3. Donor chimerism after Flu-XRT-Cy conditioning is proportional to the dose of transplanted marrow cells or TBI. / (A) B10 mice received fludarabine 100 mg/kg/day intraperitoneally on days −6 to −2, TBI (200 cGy) on day −1, graded doses of BALB/c marrow cells intravenously on day 0, and Cy 200 mg/kg intraperitoneally on day 3. (B) B10 mice received fludarabine 100 mg/kg/day intraperitoneally on days −6 to −2, graded doses of TBI on day −1, 20 million BALB/c marrow cells on day 0, and Cy 200 mg/kg intraperitoneally on day 3. (A,B) Seven weeks (closed symbols) or 1 year (open symbols) after transplantation, peripheral blood was stained with fluorescein-conjugated antibody to H-2Dd and evaluated for donor chimerism by flow cytometry.

Donor chimerism after Flu-XRT-Cy conditioning is proportional to the dose of transplanted marrow cells or TBI.

(A) B10 mice received fludarabine 100 mg/kg/day intraperitoneally on days −6 to −2, TBI (200 cGy) on day −1, graded doses of BALB/c marrow cells intravenously on day 0, and Cy 200 mg/kg intraperitoneally on day 3. (B) B10 mice received fludarabine 100 mg/kg/day intraperitoneally on days −6 to −2, graded doses of TBI on day −1, 20 million BALB/c marrow cells on day 0, and Cy 200 mg/kg intraperitoneally on day 3. (A,B) Seven weeks (closed symbols) or 1 year (open symbols) after transplantation, peripheral blood was stained with fluorescein-conjugated antibody to H-2Dd and evaluated for donor chimerism by flow cytometry.

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