Fig. 5.
![Fig. 5. JRFL gp120 induces MAPK activation in macrophages. / MDMs were stimulated with JRFL gp120 (2.5 μg/mL) or MIP-1β (200 nM) for the indicated time periods. Cell lysates were resolved by 12% SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to immunoblot analysis with antibodies specific for the total or phosphorylated forms of (A) p38 MAPK (phospho-p38 MAPK [T183/Y185] antibody); (B) JNK/SAPK (phospho-JNK/SAPK [T180/Y182] antibody); and (C) ERK1/2 (p44/42) (phospho-p44/42 MAPK [T202/Y204] antibody). Blots shown are from one donor and are representative of 5 experiments using different donors that yielded similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/98/10/10.1182_blood.v98.10.2909/5/h82211757005.jpeg?Expires=1735815240&Signature=J9lPoZzotfY5UyFSH94OmzJolrOMOG8eOK9Je7CtDLc3-Jgv8EUzc7WF4oujmOENCRtUKKfiVhMGLl~NM6qs56YnJRYeVfen7lhDNT1UPaV9C21kViEZGFQZwVVfWbNPK6TG3jruF4XWbDYix9br~4wxfOO-AWezMpttLXeUfP-dD~-lbouhg0vSNnAAOL7JYXWcntacSoMDbdpunzYNHZk~5aaAUzk9bvmc8kkMiDkwMndHKGKD50CJIpFgFV6TnNO2KB~SCAT-Asj8fPDJE8sW~Igb~k-JyVwPqXmcNu8~MqvBdZmySvY4Qr8~jBxb97VyMUScQMyIj9SpDSTUUw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
JRFL gp120 induces MAPK activation in macrophages.
MDMs were stimulated with JRFL gp120 (2.5 μg/mL) or MIP-1β (200 nM) for the indicated time periods. Cell lysates were resolved by 12% SDS-PAGE, transferred to a nitrocellulose membrane, and subjected to immunoblot analysis with antibodies specific for the total or phosphorylated forms of (A) p38 MAPK (phospho-p38 MAPK [T183/Y185] antibody); (B) JNK/SAPK (phospho-JNK/SAPK [T180/Y182] antibody); and (C) ERK1/2 (p44/42) (phospho-p44/42 MAPK [T202/Y204] antibody). Blots shown are from one donor and are representative of 5 experiments using different donors that yielded similar results.
