MAPK activation is involved in gp120-induced chemokine secretion.

MDMs were preincubated for 1 hour with or without the specific MAPK inhibitor SB-202190 (1 μg/mL) and then exposed to JRFL gp120 (1 μg/mL). After 24 hours of culture, supernatants were harvested. Levels of MIP-1β (A) and MCP-1 (B) and in the clarified supernatants were then detected by ELISA. Data are the mean of duplicate samples ± SD and one representative experiment of 4 is shown.