PLD expression profiles in U937 cells.

(A) RT-PCR was performed with mRNA extracted from untreated, IFN-γ–primed and dbcAMP-differentiated U937 cells. Specific primers for PLD1 (which yield 2 fragments corresponding to PLD1a, 640bp, and PLD1b, 520bp) and primers specific for PLD2 amplifying a 450-bp fragment, were used. The results shown are typical from 3 separate experiments. (B) Western blot analysis of immunoprecipitates of PLD1 or PLD2, from cell lysates from untreated, IFN-γ–primed and dbcAMP-differentiated U937 cells, were resolved by SDS/PAGE 8% polyacrylamide gels. Proteins were transferred to nitrocellulose and probed with anti-PLD1 or anti-PLD2 antibodies. The results shown are typical from 3 separate experiments. Mw indicates molecular weight.