Morphologic features of IFN-DCs.

Scanning electron microscopy analysis of IFN-DCs (A) showed a number of typical dendriticlike protrusions, not detected in IL-4-DCs (B), that were well adhering to the substrate. Immunocytochemistry for CD44 in IFN-DCs (C) and IL-4-DCs (D) (peroxidase-antiperoxidase method and hematoxylin counterstaining; magnification ×1500). Note the thin and long dendrites of IFN-DCs as compared with the squat dendrites of the IL-4-DCs. The CD44 staining is typically localized on dendrites, nicely outlining them.