Fig. 3.
CCR5 expression and migratory response to β-chemokines by IFN-DCs.
(A) Dot histogram FACS profiles showing CCR5 and CXCR4 staining of IFN-DCs and IL-4-DCs. (B) DCs (5 × 105), treated for 3 days with the indicated cytokines, were resuspended in complete medium and seeded in the upper compartments of 8-μm pore size filter Transwell chambers, while 0.5 μg/mL of the relevant chemokine in serum-free medium was added to the lower compartments. The lower wells of control chambers contained medium alone. After 2 hours of incubation, the cells that migrated to the lower compartment were counted. Assays were performed in triplicate. SDs did not exceed 15%. Results of one representative experiment of 4 are shown.
