Fig. 6.
PI3-K inhibition blocks BCR-mediated survival signals in CLL.
Freshly isolated CLL B cells were placed in culture with media only or with anti-IgM without or upon preincubation with the PI3-K inhibitor LY294002. (A) Apoptosis was measured by the PI method in the treated cells of 4 cases. Error bars indicate the SEM. (B) Phospho-Akt (thr 308, ser 473) was determined by western blot analyses with lysates taken at the t0 and 1 hour timepoints and specific antibody to the phosphorylated and total forms of Akt. (C) Mcl-1 protein was evaluated using lysates from t0 and 24 hours. The blot was stripped and probed also for bcl-2 and actin.
