Generation of killer DC-DC hybrids.
(A) The working model is illustrated in this schema. CD4+and CD8+ T cells from a recipient (blue) or a donor (red) recognize intact MHC molecules presented by allogeneic DCs (ie, direct presentation; indicated by d) or MHC-derived peptide antigens () presented by syngeneic DCs (ie, indirect presentation; indicated by i) (left panel). Control DC-DC hybrids expressing both recipient- and donor-derived MHC molecules should activate all of the above alloreactive T-cell populations (middle panel), whereas CD95L-transduced killer DC-DC hybrids expressing CD95L molecules (indicated by arrows) should deliver apoptotic signals to all T-cell populations (right panel). (B) Schematic flow chart of the methods developed for the generation of killer DC-DC hybrids (see text for details). (C) The indicated cell lines were examined for their proliferative potential in the presence of HAT and/or G418. Data are representative of 2 independent experiments, showing the means ± SD (n = 3) of the relative 3H-thymidine uptake (percentage of control) as compared with control culture in the absence of either HAT or G418.
