American Society of Hematology

Fig. 3.

$Fig. 3. In vitro impact of killer DC-DC hybrids on allogeneic immune responses. / (A) Control or CD95L-transduced DC-DC hybrids were γ-irradiated and cocultured with unfractionated spleen cells isolated from A/J or BALB/c mice in the presence of PBS alone, anti-CD95L mAb (Kay-10), or control IgG. (B) γ-irradiated control or killer DC-DC hybrids were added to the 2-way MLR in which unfractionated spleen cells isolated from A/J and BALB/c mice were cocultured in the absence of γ-irradiation. (C,D) γ-irradiated control or killer DC-DC hybrids were added to the one-way MLR in which CD4+ or CD8+ T cells purified from the indicated strain were cocultured with γ-irradiated splenic DCs isolated from the indicated allogeneic strain. (E) Control DC-DC hybrids (open circles) or killer DC-DC hybrids (closed circles) were examined for their cytotoxicity against A/J T cells reactive to BALB/c-associated alloantigens (left panel) or BALB/c T cells reactive to A/J-associated alloantigens (right panel) in a standard 6-hour3H-release assay at the indicated E/T ratios. All of the data in this figure are representative of at least 2 independent experiments, showing the means ± SD from triplicate samples. (*P < .01; brackets indicate groups being compared.)$

In vitro impact of killer DC-DC hybrids on allogeneic immune responses.

(A) Control or CD95L-transduced DC-DC hybrids were γ-irradiated and cocultured with unfractionated spleen cells isolated from A/J or BALB/c mice in the presence of PBS alone, anti-CD95L mAb (Kay-10), or control IgG. (B) γ-irradiated control or killer DC-DC hybrids were added to the 2-way MLR in which unfractionated spleen cells isolated from A/J and BALB/c mice were cocultured in the absence of γ-irradiation. (C,D) γ-irradiated control or killer DC-DC hybrids were added to the one-way MLR in which CD4⁺ or CD8⁺ T cells purified from the indicated strain were cocultured with γ-irradiated splenic DCs isolated from the indicated allogeneic strain. (E) Control DC-DC hybrids (open circles) or killer DC-DC hybrids (closed circles) were examined for their cytotoxicity against A/J T cells reactive to BALB/c-associated alloantigens (left panel) or BALB/c T cells reactive to A/J-associated alloantigens (right panel) in a standard 6-hour³H-release assay at the indicated E/T ratios. All of the data in this figure are representative of at least 2 independent experiments, showing the means ± SD from triplicate samples. (*P < .01; brackets indicate groups being compared.)

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