Fig. 2.
Cellular distribution of TF mRNA in tissues from lean and obese (ob/ob) mice.
In situ hybridization was performed on sections of paraffin-embedded tissues from lean mice (A,C,E,G) and obese mice (B,D,F,H) using35S-labeled TF riboprobes, as described in “Materials and methods.” Representative sections are shown. (A,B) Lung; (C,D) heart; (E,F) aorta; (G,H) liver. Arrowheads indicate examples of positive hybridization signals, and arrows indicate the absence of a hybridization signal. Slides were exposed for 4 weeks at 4°C and stained with hematoxylin and eosin. Original magnification is × 400 for all sections.
