Fig. 3.
Cellular distribution of TF mRNA in an artery in the perinephric fat from lean and obese (ob/ob) mice.
In situ hybridization was performed on paraffin-embedded perinephric fat from lean (A) and obese (B) mice using 35S-labeled TF riboprobes, as described in “Materials and methods.” Arrowheads indicate examples of positive hybridization signals, and arrows show the absence of hybridization signal in endothelial cells. Slides were exposed for 4 weeks at 4°C and stained with hematoxylin and eosin. Original magnification is × 400 for both sections.
