PI3K phosphorylation by TEL/PDGFβR.

PI3K tyrosine phosphorylation and association with TEL/PDGFβR fusion proteins in Ba/F3 cells. (A) Ba/F3 cell lysates were immunoprecipitated with antibody against the 85-kd subunit of PI3K and immunoblotted with antibody against phosphotyrosine. (B) PI3K immunoprecipitates were immunoblotted with antibody against p85^PI3K to assess relative expression and immunoprecipitation. (C) Ba/F3 cells were immunoprecipitated with antibody against the PDGFβR cytoplasmic domain and immunoblotted with antibody against p85^PI3K.