American Society of Hematology

Fig. 3.

$Fig. 3. Redox cycling of DBBF-Hb induces cell cycle arrest in the G2/M phase. / Cells were incubated with FBS-free medium alone (control) or medium containing 2 mU/mL GOX with or without 50 μM DBBF-Hb. At each time interval, adherent and nonadherent cells were pooled, and fixed. DNA content was determined by flow cytometry after RNase digestion and PI staining. The fraction of cells in the (A) G1 phase, (B) S phase, and (C) G2/M phase of the cell cycle was calculated as described in “Materials and methods.” Each point represents the mean ± SE for 3 to 6 independent experiments. *P < .05 versus control. (D) Ferryl hemoglobin formation in medium containing DBBF-Hb with or without 2 mU/mL GOX. Ferryl hemoglobin was measured using the sodium sulfide method as described in “Materials and methods.” Each point represents the mean ± SE for 4 to 5 samples.$

Redox cycling of DBBF-Hb induces cell cycle arrest in the G2/M phase.

Cells were incubated with FBS-free medium alone (control) or medium containing 2 mU/mL GOX with or without 50 μM DBBF-Hb. At each time interval, adherent and nonadherent cells were pooled, and fixed. DNA content was determined by flow cytometry after RNase digestion and PI staining. The fraction of cells in the (A) G1 phase, (B) S phase, and (C) G2/M phase of the cell cycle was calculated as described in “Materials and methods.” Each point represents the mean ± SE for 3 to 6 independent experiments. *P < .05 versus control. (D) Ferryl hemoglobin formation in medium containing DBBF-Hb with or without 2 mU/mL GOX. Ferryl hemoglobin was measured using the sodium sulfide method as described in “Materials and methods.” Each point represents the mean ± SE for 4 to 5 samples.

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