Redox cycling of DBBF-Hb alters intracellular thiol status.

(A) Soluble reduced thiols in endothelial cells were measured after a 4-hour incubation with 50 μM DBBF-Hb and GOX (2 or 10 mU/mL) in the presence or absence of 100 U/mL catalase. Values are reported as the means ± SE for 2 to 5 independent experiments performed in triplicate. *P < .05 versus control. (B) Soluble reduced thiols were measured after incubation with 10 mU/mL GOX and DBBF-Hb (0-100 μM). Values are reported as the means ± SE for 2 to 5 independent experiments performed in triplicate. (C) Time course for reduced thiol depletion in endothelial cells by BSO, an inhibitor of GSH synthesis. Cells were incubated in complete EGM medium with or without 1 mM BSO. Values were recorded as the percentage of control cells (medium alone) for each experiment and were reported as the means ± SE for 3 to 5 independent experiments performed in triplicate.