Prior depletion of GSH exacerbates G2/M arrest and apoptosis.

Normal and BSO-pretreated cells were incubated with FBS-free medium alone or with medium containing 50 μM DBBF-Hb and 10 mU/mL GOX. At each time interval, adherent and nonadherent cells were pooled and analyzed for (A) DNA content and (B) PS externalization. No differences in DNA content or PS externalization were observed between media-treated normal and BSO cells. Values at each time point are reported as the mean ± SE of 3 to 6 independent experiments. *P < .05 versus DBBF-Hb and GOX in normal cells.