Detection of donor cells in the bone marrow of mice that received transplanted cells.

Bone marrow cells were not covered (A) or were covered (B) with PMPs, were isolated from animals receiving transplanted cells, and subsequently were stained with α-Ly5 monoclonal antibodies. Because donor and host mice differed in the Ly5 locus, donor and host cells were easily detectable by Ly5.1- or Ly5.2-specific monoclonal antibodies. A representative study of 2 is shown.