Fig. 3.
PTPεC inhibits IL-6–induced activation of STAT1.
M1 stable clones were treated with IL-6 at 10 ng/mL or with IFN-γ at 25 ng/mL for 20 minutes and lysed. Phosphorylated STAT1 was detected by Western blotting with an antibody specific to tyrosine-phosphorylated STAT1 (upper panels). Blots were stripped and reprobed with the anti-STAT1 antibody (lower panels).