Restriction digestion patterns of PCR products detect mutations in the

URO-D gene. Genomic DNA from probands and controls was PCR amplified, digested with a restriction enzyme, and separated on a 2% agarose gel. Lanes are marked as either mutant or control (above), and the restriction enzyme used is indicated below the gel. A lane containing an undigested PCR product (control) is also included. Molecular markers are present on the outside lanes of each gel.