Fig. 8.
Fig. 8. Overexpression of p21 in murine megakaryocytes. / Lin− cells from wild-type CF1 or p21−/− bone marrow were cultured for 2 days in the presence of PEG-rHuMGDF and SCF and infected with an ecotropic retrovirus containing either the HA-tagged p21 and EGFP cDNA (panels B, D) or EGFP cDNA alone vectors (panels A, C) (3 experiments). After infection, more than 50% of the megakaryocytes expressed EGFP as assessed by microscopy. Because the FITC anti-CD41 mAb could not be used for cell sorting, ploidy was measured by flow cytometry in cells with at least 8N. Overexpression of p21 significantly decreased the mean ploidy of megakaryocytes in comparison with cells infected with the control vector independent of p21 status of the mice (CF1, panels A, B; p21−/−, panels C, D). Similar results were obtained in C57Bl/6 mice (data not shown, n = 3).

Overexpression of p21 in murine megakaryocytes.

Lin− cells from wild-type CF1 or p21−/− bone marrow were cultured for 2 days in the presence of PEG-rHuMGDF and SCF and infected with an ecotropic retrovirus containing either the HA-tagged p21 and EGFP cDNA (panels B, D) or EGFP cDNA alone vectors (panels A, C) (3 experiments). After infection, more than 50% of the megakaryocytes expressed EGFP as assessed by microscopy. Because the FITC anti-CD41 mAb could not be used for cell sorting, ploidy was measured by flow cytometry in cells with at least 8N. Overexpression of p21 significantly decreased the mean ploidy of megakaryocytes in comparison with cells infected with the control vector independent of p21 status of the mice (CF1, panels A, B; p21−/−, panels C, D). Similar results were obtained in C57Bl/6 mice (data not shown, n = 3).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal