TRAIL-induced apoptosis in MDS marrow.

TRAIL-induced apoptosis in aberrant MDS blasts (A) and in CD34⁺ precursors from MDS marrow (B). Panels in the left columns represent data before, and in the right columns, after TRAIL exposure. Shown are the gates used (top), scattergrams (middle), and DNA histograms (bottom) of gated cells. FSC indicates forward scatter; SSC, orthogonal scatter; FLI-FITC, fluorescence intensity after labeling with FITC-conjugated Annexin V; FL2-PI, fluorescence intensity after staining with propidium iodine; counts, relative number of cells. Percentage figures in the bottom panels indicate proportions of apoptotic cells.