TFPI and apo(a) immunostaining coexist in human coronary atherosclerotic plaque.

(A) Photomicrograph showing hematoxylin and eosin staining of human fibrocellular plaque obtained by coronary atherectomy (objective × 20). (B) Adjacent section showing SMC α-actin staining (peroxidase with DAB substrate, objective × 20). (C) Adjacent section showing apo(a) immunostaining (alkaline phosphatase with vector blue, objective × 20). (D) Adjacent section showing TFPI immunostaining (alkaline phosphatase with fast-red substrate, objective × 20). (E) Adjacent section showing double immunostaining of TFPI (red) and apo(a) (blue) (objective × 20). (F) Adjacent section showing lack of staining by mouse anti-PLG antibody (objective × 20). (G-I) Adjacent sections showing lack of staining by isotype-matched mouse, rabbit, and goat IgG control antibodies (objective × 20).