Effect of hypoxia on TGF-β2 message stability.

HUVECs subjected to normoxic conditions, C, or to 24 hours of hypoxia were treated with 1 μg/mL α-amanitin for the times indicated. (A) TGF-β2 message obtained with RNase protection analysis in HUVECs subjected to hypoxia for 24 hours and subsequently treated with α-amanitin for the times indicated. In parallel cultures, normoxic HUVECs were also cultured with and without α-amanitin (not shown except for the 0-hour control loaded in the first lane). (B) Semilog plot of TGF-β2 transcript half-life in the presence of hypoxic or normoxic conditions. Data were quantitated by PhosphorImager and the results plotted with linear regression; data from 1 of the 2 representative experiments are shown.