Prostratin up-regulates TNF-α expression in treated PBMCs.

Human PBMCs were treated with mitogenic or proliferative agents as indicated. TNF-α release into the culture media was measured by ELISA and peaked by day 2 after treatment for cultures exposed to prostratin, PHA/IL-2, or PHA alone. IL-2 and DMSO control cultures elicited no significantly greater effect in TNF-α production than no addition. Each point represents the mean of 2 trials ± 1 SD.