Gene array analysis of prostratin-treated THP-1 cells.

Total RNA was isolated from untreated THP-1 cells or THP-1 cells exposed to 0.3 μM prostratin for 24 hours. Radiolabeled cDNA was synthesized for each RNA sample and hybridized to duplicate array filters. Images were captured by a phosphoimager analysis after overnight exposure and array values normalized as indicated in “Materials and methods.” Each horizontal doublet represents the positions of genes analyzed for expression level differences. Long arrows denote positions of IL-1β and short arrows denote positions for MMP-9 and are scribed to illustrate differences of the primary data as specific examples.