Prostratin induced down-regulation of CD4 on isolated total T-helper (Th) CD4⁺ and CD4⁺ resting Th cells.

CD4⁺ and CD4⁺HLA-DR⁻ T cells were isolated from HIV-1–seronegative donors via negative selection and exposed in duplicate to doses of 0, 0.1, 0.3, 1.0, and 1.5 μM prostratin for 48 hours. The cells were then double-stained for CD3 and CD4. The cells were gated for CD3 and analyzed for mean CD4 expression above background. Each point reflects the mean of 2 trials ± 1 SD.