Prostratin induction of HIV-1 expression from HAART patient PBMCs.

CD8⁺ T cell–depleted PBMCs from HAART patients were treated with a variety of mitogenic or proliferative agents either alone or in combination (10 μg/mL PHA with 50 IU/mL IL-2, or 0.263 μM prostratin) as indicated and cocultured with HIV-1–seronegative donor CD8⁺ T cell–depleted PBMCs as described in “Materials and methods.” Media was withdrawn to determine p24 antigen content by ELISA once or twice a week as indicated. Boxed insets represent an amplified view of graph region below.