BLIN-3 cells express TdT.

(A) Analysis of TdT mRNA expression in μ⁻ BLIN-3 and μ⁺ BLIN-3 cells by RT-PCR followed by Southern blot analysis. BLIN-2 is a TdT⁺ pre-B ALL line included as a positive control. H₂O, no template; RT−, no reverse transcriptase. (B) Analysis of TdT protein expression in μ⁻ BLIN-3 and μ⁺ BLIN-3 cells. BLIN-2 is included as a positive control. The bar labeled M1 denotes staining by the isotype-matched control. GAPDH indicates glyceraldehyde phosphate dehydrogenase.