Fig. 5.
Fig. 5. BLIN-3 cells do not express immunoglobulin light-chain proteins. / (A) RT-PCR analysis of rearranged kappa transcripts in μ− and μ+ BLIN-3. cDNA was PCR amplified using a Vκ consensus primer and a Cκ primer followed by Southern blot analysis, as described in “Materials and methods.” (B) Intracellular flow cytometric analysis of κ and λ protein expression in BLIN-3 sublines. DAUDI cells are included as a positive control for κ, and RAMOS cells are included as a positive control for λ. The bar labeled M1 denotes staining by the isotype-matched control. Cyto indicates cytoplasmic.

BLIN-3 cells do not express immunoglobulin light-chain proteins.

(A) RT-PCR analysis of rearranged kappa transcripts in μ− and μ+ BLIN-3. cDNA was PCR amplified using a Vκ consensus primer and a Cκ primer followed by Southern blot analysis, as described in “Materials and methods.” (B) Intracellular flow cytometric analysis of κ and λ protein expression in BLIN-3 sublines. DAUDI cells are included as a positive control for κ, and RAMOS cells are included as a positive control for λ. The bar labeled M1 denotes staining by the isotype-matched control. Cyto indicates cytoplasmic.

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