Fig. 8.
Fig. 8. Acquisition of μ in BLIN-3 sublines is associated with V to DJ rearrangement. / (A) Analysis of DJ rearrangements in BLIN-3 sublines. Genomic DNA from BLIN-3 sublines expressing different levels of surface μ was amplified by PCR using a 5′ DXP-DH element primer and a 3′ JH6 primer. The JH designations on the right side of the blot define the sizes of amplified fragments representing specific JH family members. (B) Analysis of VH6-family VDJ rearrangements in BLIN-3 sublines. Genomic DNA from BLIN-3 sublines expressing different levels of surface μ was analyzed for VDJ rearrangements using a VH6 family-specific 5′ primer and a JH6 3′ primer. BLIN-3 sublines bearing higher percentages of μ+ cells exhibited more VDJ rearrangements. In panels A and B, the RAG-1 gene was amplified as a loading control. H2O, no DNA template.

Acquisition of μ in BLIN-3 sublines is associated with V to DJ rearrangement.

(A) Analysis of DJ rearrangements in BLIN-3 sublines. Genomic DNA from BLIN-3 sublines expressing different levels of surface μ was amplified by PCR using a 5′ DXP-DH element primer and a 3′ JH6 primer. The JH designations on the right side of the blot define the sizes of amplified fragments representing specific JH family members. (B) Analysis of VH6-family VDJ rearrangements in BLIN-3 sublines. Genomic DNA from BLIN-3 sublines expressing different levels of surface μ was analyzed for VDJ rearrangements using a VH6 family-specific 5′ primer and a JH6 3′ primer. BLIN-3 sublines bearing higher percentages of μ+ cells exhibited more VDJ rearrangements. In panels A and B, the RAG-1 gene was amplified as a loading control. H2O, no DNA template.

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