Lymphocyte activation and CD95 sensitivity during chemotherapy.

PBLs were analyzed for expression of CD69 (A, B) and sensitivity for CD95-induced apoptosis (C, D) before the first (1st) and consecutive (2nd/3rd) cycles of chemotherapy (A, C) and during the acute phase of lymphocyte depletion of the first cycle (B, D). Percentage of CD69⁺ cells of CD4 cells is depicted (A, B). MNCs were cultured in medium with and without death-inducing anti-CD95. Percentage of CD4 cells positive for annexin V was measured after 24 hours, and the percentage of CD95-specific cell death is given in the graphs (B, D).