Induction of mitochondrial membrane permeability transition in CD4 and CD8 T cells by in vivo chemotherapy.
Patients' CD4 and CD8 lymphocytes were analyzed by flow cytometry for lowered uptake of DiOC6(3) indicating inner mitochondrial membrane permeability transition. (A) Contour plots of a representative analysis (patient A10). Fifty thousand cells were acquired in the lymphoid gate. Gating for CD4 and CD8 was performed in the side scatter versus fluorescence plots. Percentages represent the proportion of cells with low mitochondrial membrane potential. (B) For patients A3 to A10, the number of cells with low mitochondrial membrane potential (ΔΨ low) was assessed. The graph depicts pretreatment values (0 hour) and values after 48 hours of chemotherapy for individual patients.
