A rescued immunoglobulin from an HCV-associated lymphoma binds soluble E2₆₆₁.

(A) An anti-E2 mAb, CBH-4G (▾),27 HCV-associated immunoglobulin (patient 1, ⋄), HCV-associated immunoglobulin (patient 2, ○) and non-HCV lymphoma immunoglobulin controls (n = 23; ▪) were serially diluted and added to E2₆₆₁-coated wells, and bound immunoglobulin was determined by ELISA. (B) Supernatants from AdV-infected HeLa cells expressing HCV-E2₆₆₁ (lanes 1, 3, and 5) or LacZ (lanes 2, 4, and 6) were immunoprecipitated with immunoglobulins derived from an anti-E2 mAb (CBH-4G) and from the 2 HCV-associated lymphoma cases. Proteins were separated by SDS-PAGE, and blots were probed with an antibody against HCV-E2. Sizes (in kd) of protein markers are indicated on the right, and the bracket on the left indicates the precipitated heterogeneously glycosylated E2₆₆₁ protein.