RT-PCR analysis.

(A) RT-PCR analysis with primers E1+ and FGFR9− show the presence of a specific band in patients 1 and 2, but not in 4 control patients with CML (C1-C4); B indicates blank; M, 123-bp ladder. (B) In-frame fusions of BCR-FGFR1 and FGFR1-BCR. FGFR1 sequences are underlined. (C) Structure of BCR-FGFR1 in which BCR amino acid 584 is fused to FGFR1 amino acid 429. The chimeric protein is predicted to contain the BCR oligomerization domain (OD), the BCR Ser/Thr kinase domains, and Y177 Grb2-binding site joined to part of the FGFR1 juxtamembrane domain and the entire FGFR1 tyrosine kinase and C-terminal domains.